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legendplex cloud based data analysis software  (Qognit Inc)

 
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    Structured Review

    Qognit Inc legendplex cloud based data analysis software
    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by <t>LEGENDplex.</t> Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
    Legendplex Cloud Based Data Analysis Software, supplied by Qognit Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloud-based+data+analysis+software/analysis+based+cloud+data+legendplex+software/pmc13171165-609-4-9
    Average 86 stars, based on 1 article reviews
    legendplex cloud based data analysis software - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Human LFA-1 governs T cell immune surveillance of the skin"

    Article Title: Human LFA-1 governs T cell immune surveillance of the skin

    Journal: Science immunology

    doi: 10.1126/sciimmunol.adz8360

    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
    Figure Legend Snippet: Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Techniques Used: Flow Cytometry, MANN-WHITNEY, Immunopeptidomics

    Related Articles

    Software:

    Article Title: In vitro bioactive dentin protein release by diode laser conditioning.
    Article Snippet: Dentin is the largest structure in the tooth, intimately connected to the pulp tissue, forming an embryological and functional association known as the pulp–dentin complex [1].. Approximately 20% of its weight is organic components of the extracellular matrix, mainly composed of typeI collagen and noncollagenous matrix proteins, including glycoproteins, proteoglycans, enzymes, phospholipids, and various growth factors.. Differentiated odontoblasts secrete the extracellular matrix during development, providing a structural framework for apposition minerals [1,2].

    Article Title: Platelet-Derived Microvesicles Modulate Cytokine and Lipid Mediator Profiles in THP-1 Monocytes and Macrophages.
    Article Snippet: Cytokine release was measured using flow cytometry with the LEGENDplex Human Inflammation Panel 1 (BioLegend), which allows the quantification of IL-1β, interferon (IFN)-α2, IFN-γ, tumor necrosis factor (TNF)-α, macrophage chemoattractant protein (MCP)-1, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, IL-23 and IL-33, following the manufacturer’s protocol. .. The results were analyzed using the cloud-based LEGENDplex data analysis software (Qognit). .. Samples were initially fixed in 2.5% glutaraldehyde (Sigma-Aldrich) in PBS at 4°C for 2 hours, followed by post-fixation in 1% osmium tetroxide (OsO4; Sigma-Aldrich) at 4°C for an additional 2 hours.

    Article Title: Human LFA-1 governs T cell immune surveillance of the skin
    Article Snippet: .. Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (Qognit). .. Cytokine concentrations were determined in multiplex bead assays with Human T Helper Cytokine Panel Version 2 (BioLegend, catalog no. 741028).

    Article Title: Pre-Vaccination Immune Profiles and Responsiveness to Innate Stimuli Predict Reactogenicity and Antibody Magnitude Following mRNA Vaccination
    Article Snippet: After a 1 h room temperature incubation in the dark with shaking, streptavidin–PE was added without an intermediate wash. A final 30 min incubation was immediately followed by an additional wash. Wells were resuspended in additional wash buffer, and samples were acquired on the Cytek Aurora Spectral Cytometer (Cytek Biosciences, Fremont, CA, USA). .. Data were analyzed using the cloud-based Qognit data analysis software (Qognit, San Carlos, CA, USA). ..

    other:

    Article Title: Contribution of parasite and host genotype to immunopathology of schistosome infections.
    Article Snippet: The analysis was conducted using LEGENDplexTM Cloud-based Data Analysis Software (https:// legen dplex. qognit. com/).

    Article Title: Analysis of human colostrum reveals differential co-occurrence networks of metabolites, microbiota and cytokines in maternal obesity.
    Article Snippet: Data analysis was performed using BD software, which is accessible online at the website of LEGENDplexTM cloud-based data analysis software (https://legendplex.qognit.com).

    Article Title: Contribution of parasite and host genotype to immunopathology of schistosome infections
    Article Snippet: The analysis was conducted using LEGENDplexTM Cloud-based Data Analysis Software ( https://legendplex.qognit.com/ ).

    Analysis:

    Article Title: Pre-Vaccination Immune Profiles and Responsiveness to Innate Stimuli Predict Reactogenicity and Antibody Magnitude Following mRNA Vaccination
    Article Snippet: After a 1 h room temperature incubation in the dark with shaking, streptavidin–PE was added without an intermediate wash. A final 30 min incubation was immediately followed by an additional wash. Wells were resuspended in additional wash buffer, and samples were acquired on the Cytek Aurora Spectral Cytometer (Cytek Biosciences, Fremont, CA, USA). .. Data were analyzed using the cloud-based Qognit data analysis software (Qognit, San Carlos, CA, USA). ..



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    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Journal: Science immunology

    Article Title: Human LFA-1 governs T cell immune surveillance of the skin

    doi: 10.1126/sciimmunol.adz8360

    Figure Lengend Snippet: Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Article Snippet: Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (Qognit).

    Techniques: Flow Cytometry, MANN-WHITNEY, Immunopeptidomics